Flow Cytometry in Hematolymphoid Neoplasms. A Comprehensive and Practical Guide to Diagnosis
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Flow Cytometry in Hematolymphoid Neoplasms. A Comprehensive and Practical Guide to Diagnosis

Sa A. Wang, Wei Wang
SKU: 9781009397766
ISBN: 9781009397766
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Περιγραφή

Flow cytometry plays a critical role in the diagnosis of hematopoietic neoplasms. Understanding the key immunophenotypic features of each entity is essential for accurate diagnosis. Filling a crucial gap in current literature, this is a comprehensive reference text that systematically details these immunophenotypic profiles. Grounded in real-world experience, this practice-driven resource offers expert guidance on the use of flow cytometry in the diagnosis and classification of hematolymphoid neoplasms. Covering major disease categories, technical insights, and emerging therapies like CAR T-cells, readers will gain a solid foundation for identifying deviations seen in hematologic malignancies, thereby enhancing diagnostic accuracy. Each chapter concludes with key points and diagnostic pitfalls for efficient comprehension and retention. This is an essential tool for pathologists, hematologists, trainees, and laboratory professionals seeking clarity and confidence in diagnosis.

  • Key points and diagnostic pitfalls are summarized in a table format at the end of each chapter, allowing readers to quickly grasp the chapter content and identify the key learning points
  • Differential diagnosis is discussed in each chapter, helping readers distinguish between entities with overlapping immunophenotypic features by highlighting key differences, thereby avoiding misdiagnosis
  • For each hematopoietic lineage, chapters cover normal maturation pattern and immunophenotype, followed by a description of the abnormal immunophenotypic features associated with aberrancies. Understanding this provides a strong foundation for recognizing deviations seen in hematologic malignancies, enhancing diagnostic accuracy
  • Diagnostic pitfalls and interpretive caveats are commonly encountered in flow cytometric analysis

Περιεχόμενα

Section I. Mature B-Cell and Plasma Cell Neoplasms

1. B-Cells: normal maturation and strategies in identification of abnormal cells Wei Wang

2. Chronic lymphocytic leukemia/small lymphocytic lymphoma and mantle cell lymphoma Wei Wang

3. Follicular lymphoma Wei Wang

4. Low-grade b-cell lymphomas with plasmacytic differentiation Andres E Quesada and Pei Lin

5. Splenic b-cell lymphoma/leukemia (other than splenic marginal zone lymphoma) and b-cell prolymphocytic leukemia Beenu Thakral and Sa A Wang

6. Aggressive b-cell lymphomas Shaoying Li, Jie Xu and Lianqun Qiu

7. Large b-cell lymphoma with plasmablastic differentiation Lianqun Qiu, Shaoying Lin, Pei Lin, Jiehao Zhou, Yi Zhou and Jie Xu

8. Plasma cell neoplasms Andres E Quesada and Pei Lin

Section II. Mature T-Cell and Nk-Cell Neoplasms

9. T and NK cells: normal maturation and strategies in identification of abnormal cells Wei Wang

10. T-cell large granular lympocytic leukemia Min Shi, Michael M. Timm and Dragan Jevremovic

11. Hepatosplenic T-cell lymphoma Min Shi, Michael M. Timm and Dragan Jevremovic

12. Adult T-cell leukemia/lymphoma Hong Fang and Wei Wang

13. T-prolymphocytic leukemia Hong Fang and Wei Wang

14. Mycosis fungoides/ Sezary syndrome Hong Fang and Wei Wang

15. NK-Large granular lympocytic leukemia Min Shi, Michael M. Timm and Dragan Jevremovic

16. Aggressive NK-cell leukemia Min Shi, Michael M. Timm and Dragan Jevremovic

17. Nodal T follicular helper cell lymphoma Chi Young Ok, Francisco Vega and Sa A. Wang

18. Anaplastic large cell lymphoma, ALK-positive Jie Xu, Shaoying Li and L. Jeffrey Medeiros

19. Anaplastic large cell lymphoma, ALK-negative Jie Xu, Jeffrey Medeiros and Shaoying Li

20. Other nodal and extranodal mature T/NK-cell lymphomas Chi Young Ok, Francisco Vega and Sa A. Wang

Section III. Acute Lymphoblastic Leukemia/Lymphoma

21. B-lymphopblastic leukemia/lymphoma Sa A. Wang

22. T-lymphopblastic leukemia/lymphoma Sa A. Wang

Section IV. Acute Myeloid Leukemia

23. Acute myeloid leukemia: introduction Wei Wang

24. Acute myeloid leukemia with RUNX1: RUNX1T1 fusion Wei Wang

25. Acute myeloid leukemia with CBFB rearrangement Wei Wang

26. Acute promyelocytic leukemia Hong Fang and Wei Wang

27. Acute myeloid leukemia with NPM1 mutation Wei Wang

28. Acute myeloid leukemia with KMT2A rearrangement Wei Wang

29. Acute myeloid leukemia with MECOM rearrangement Wei Wang

30. Acute myeloid leukemia with DEK: NUP214 fusion Wei Wang

31. Acute myeloid leukemia with NUP98 rearrangement Mahsa Khanlari and Wei Wang

32. Acute myeloid leukemia with mutated TP53 Fnu Sameeta and Wei Wang

33. Acute erythroid leukemia Wei Wang

34. Acute megakaryoblastic leukemia Mahsa Khanlari and Wei Wang

35. Normal myelomonocytic maturation and evaluation of acute myeloid leukemia minimal/measurable residual disease Wei Wang and Sa A. Wang

Section V. Acute Leukemias of Mixed or Ambiguous Lineage

36. Acute undifferentiated leukemia Vasiliki Leventaki and Sa A. Wang

37. Mixed-phenotype acute leukemia Vasiliki Leventaki and Sa A Wang

Section VI. Neoplastic Plasmacytoid Dendritic Cell Proliferation

38. Plasmacytoid dendritic cells: from normal maturation to neoplastic proliferations Wei Wang

Section VII. Other Myeloid Neoplasms and Bone Marrow Failure Diseases

39. Chronic myeloid neoplasms and bone marrow failure disorders Sanam Loghavi, Sa A. Wang and Wei Wang

40. Systemic mastocytosis Wei Wang, Jie Xu, Sa A. Wang, Lianqun Qiu and Shaoying Li

Section VIII. Flow Cytometry Data Handling

41. Flow cytometry data processing, analysis, artifacts and pitfalls Xuehai Wang

Section IX. Chimeric Antigen Receptor (Car) T-Cell

42. Chimeric antigen receptor (CAR) T-Cell immunotherapy and flow cytometric monitoring of CAR T-cells Jianhua Ling and Sa A. Wang

Appendix: common flow cytometry markers and their utility.

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